Posts

Showing posts from October, 2022

Basics of Cell Culture- Isolation, Culture and Passage

Isolating particular cells from a living creature and then growing those cells in a laboratory using artificial medium is the procedure that is known as cell culture. Before being cultivated, the cells can either be extracted from the tissue and further processed using additional enzymatic or mechanical means, or they can be derived from an already established cell line or cell strain.  Isolating the cells from the tissue and processing them further before cultivation are both viable options. Primary culture is the stage of culture that happens when tissue-isolated cells have multiplied under ideal conditions to fill all available substrate. This stage of culture is referred to by the name "primary culture" (i.e., reached confluence). It is important at this stage to subculture (also known as "passaging") the cells into a new vessel with fresh growth medium. This gives for additional space for the cells to continue expanding and is necessary. Protecting Cell Culture...

The Link Between Age And Genetic Expression

Image
A recent study has shown that age often plays a bigger role than genetics in gene expression and susceptibility to disease. The study, which was conducted by researchers at the University of Edinburgh, looked at the effects of aging on gene expression in cells. The findings, published in the journal Nature Communications, suggest that as we age, our cells become more susceptible to diseases such as cancer. The study also found that this susceptibility is not down to our genes but rather how our cells age. This is an important discovery as it means that we may be able to treat diseases by targeting the aging process itself, rather than individual genes. This could lead to new and more effective treatments for conditions such as cancer. The study found that age plays a bigger role in gene expression and disease susceptibility than genetics. The study looked at the genes of mice and found that as the mice aged, their genes became more expressed and more susceptible to diseases. The study...

MEA reacts strongly to comments by foreign ministers of Germany & Pakistan on J&K

Image
The Ministry of External Affairs (MEA) has reacted strongly to comments made by the foreign ministers of Germany and Pakistan on the situation in Jammu and Kashmir. In a statement, the MEA said that the remarks were "unwarranted and unacceptable", and that they "interfere in India's internal affairs". The MEA also said that it was "deeply concerned" by the comments, and that it would be taking up the matter with the Pakistani and German governments. The Ministry of External Affairs (MEA) has strongly reacted to the comments made by the foreign ministers of Germany and Pakistan on Jammu & Kashmir. In a statement, the MEA said that it is "unfortunate" that the German Foreign Minister Heiko Maas chose to echo the Pakistani narrative on Jammu & Kashmir during his recent visit to Islamabad. The MEA also took exception to the comments made by Pakistani Foreign Minister Shah Mahmood Qureshi in which he had claimed that India was planning to...

Bihar CM Nitish Kumar claims Prashant Kishor had asked him to merge JD(U) with Congress

Image
In a recent turn of events, Bihar Chief Minister Nitish Kumar has claimed that political strategist Prashant Kishor had asked him to merge JD(U) with the Congress party. This comes as a shock to many, as Kishor is currently working with the BJP on their 2019 Lok Sabha election campaign.  Kumar made the claim during an interview with Indian Express, saying that Kishor had approached him in 2016 about the merger. He also said that Kishor had been pushing for the idea even after he joined the BJP. This news will likely cause a rift between Kishor and the BJP, as well as between Kumar and the JD(U). It remains to be seen how this will all play out in the coming months. The current situation in Bihar is that Chief Minister Nitish Kumar has claimed that political strategist Prashant Kishor had asked him to merge his Janata Dal (United) party with the Congress party. This claim was made in an interview with Hindustan Times, in which Kumar also said that he had rejected Kishor's request. K...