Basics of Cell Culture- Isolation, Culture and Passage
Isolating particular cells from a living creature and then growing those cells in a laboratory using artificial medium is the procedure that is known as cell culture. Before being cultivated, the cells can either be extracted from the tissue and further processed using additional enzymatic or mechanical means, or they can be derived from an already established cell line or cell strain. Isolating the cells from the tissue and processing them further before cultivation are both viable options. Primary culture is the stage of culture that happens when tissue-isolated cells have multiplied under ideal conditions to fill all available substrate. This stage of culture is referred to by the name "primary culture" (i.e., reached confluence). It is important at this stage to subculture (also known as "passaging") the cells into a new vessel with fresh growth medium. This gives for additional space for the cells to continue expanding and is necessary. Protecting Cell Culture...